POTENCY OF ETHANOL EXTRACT OF DAYAK ONION
(Eleutherineamericanamerr) AS PROTECTOR OF TESTOSTERONLEVEL,
DIAMETER AND THICKNESS OF SEMINIFEROUS TUBULE IN MICE
INDUCED WITH LEAD ACETATE

ParamitaRatna Gayatri1*, Sri Agus Sudjarwo2, Reny I'tishom3
Page No. 43-51

Lead is a heavy metal that affects the health, one of them are male reproductive organ. Lead
exposure leads to the formation of ROS (Reactive Oxygen Species) and decreases antioxidant
reserves in the body. Lead toxicity in male reproductive system can be prevented with antioxidants.
Dayak onion is a potential plant as a source of natural antioxidants.The aim of this study was to
determine the potential of dayak onion ethanol extract to maintain testosterone level, diameter and
thickness of seminiferous tubules in mice induced with lead acetate. The type of this study was
purely experimental laboratory (true experimental) study with Posttest Only Control Group design.
Subjects of the study were 30 male (Mus musculus) mice of Balb/C strains induced with lead acetate
of 0.75 mg/KgBW/day and divided into 5 treatment groups, negative control group (K0), positive
control group (K1), and treatment groups that received dayakonion extracts of 30 mg/KgBW/day
(K2), 60 mg/KgBW/day (K3), and 120 mg/KgBW/day (K4). On day 39, the mice were sacrificed for
measuring testosterone level, diameter and thickness of seminiferous tubule epithelium. Results
showed significant differences in the levels of testosterone hormone in groups K0 with K1, K1 with
K2 and K3. There was an increase in diameter of the seminiferous tubules of groups K2, K3, K4
compared to K1. Significant differences were obtained in the thickness of seminiferous tubular
epithelium between groups K0 with K1, K1 with K2, K3, K4. In conclusion, dayakonion ethanol
extracts are capable of maintaining testosterone and thickness of the seminiferous tubule
epithelium, but are unable to maintain the diameter of the seminiferous tubules induced lead acetate
Keywords: ethanol extract of dayakonion, testosteronehormone level, diameter and thickness of
seminiferous tubule, lead acetate.