In-vivo evaluation of Antihyperglycemic, Antidyslipidemic and Antioxidant activities of hydro-ethanolic Pithecellobium dulce (Roxb.) Benth. pod extract in alloxan induced diabetic swiss albino male mice

Swaati Sharma*, Nitika Verma, Veena Garg
Page No. 54-64

As medicinal plants are suitable alternatives for synthetic and chemical drugs hence this study was
performed to evaluate the role of hydro-ethanolic pod extract of Pithecellobium dulce (Roxb.)
Benth,in alloxan induced diabetic male mice by measuring glucose levels, lipid profile and
antioxidants activity in a period of 45 days treatment. Diabetes was induced in mice by using single
intraperitonial injection of alloxan monohydrate (150mg/kg body weight). All mice of one group were
orally administered by a single dose of 300mg/kg BW/day of PDPE extract whereas mice of another
diabetic group were treated with glibenclamide (10mg/kg of bw.) for 45 days. After this period of
treatment serum was used to analyze fasting blood glucose levels, and lipid profile and various
homogenates viz. liver, kidney, pancreas were used for the estimation of hepatic glycogen content and
enzymatic and non-enzymatic antioxidants such as Superoxide dismutase (SOD), Catalase (CAT),
glutathione peroxidase (GPx), reduced glutathione (GSH) and Thiobarbituric acid reactive
substances (TBARS). The effects of PDPE treatment were compared with that of reference
antidiabetic drug Glibenclamide along with diabetic and normal control (DC & NC) group. 45 days
treatment of PDPE significantly (P<0.05) lowered FBG levels in diabetic mice whereas, hepatic
glycogen content was elevated with normalized lipid profile. Moreover, the activity of enzymatic
and non- enzymatic antioxidants was normalized to such an extent that helped in reduction of
oxidative damage in the tissues of diabetic animals. The findings of the present study indicated
significant anti-hyperglycemic, antidyslipidemic and anti-oxidant activity in P.dulce pods.
Key words: Antidiabetic, Antioxidantive, Antidyslipidemic, Oxidative stress, lipid profile,
Diabetes Mellitus, Pithecellobium dulce (Roxb.) Benth.