In vitro bioefficacy of Hydro-ethanolic extracts of Ricinus communis bark and root for Antioxidative and Antihyperglycemic potential.

Veena Garg[1],Nitika Verma[2], Swaati Sharma[3]
Page No. 100-108

Hyperglycemia due to absolute or relative insulin deficiency, along with a condition of oxidative
stress is a typical characteristic of diabetes. Antioxidants play a vital role in the alleviation of these.
The present study was designed to study the in vitro antioxidant and antidiabetic potentials of the
hydro-ethanolic extracts of Ricinus communis (RC) bark and root. The models used for the
antioxidant ability were non enzymatic (DPPH radical scavenging assay, Hydroxyl Radical
Scavenging assay, Reducing power assay) and enzymatic antioxidants (Catalase(CAT), Superoxide
Dismutase(SOD) and Peroxidase(Px)) whereas, antidiabetic potential was estimated by measuring
the α-amylase and α-glucosidase activities. Both the extracts exhibited a dose dependent increase in
the percentage inhibition and enzyme activities thus, showing an increment in the presence of
antioxidants and antidiabetic activity with rising concentration. RC root exhibited IC50 values of
401 μg/ml for α-amylase and 598 μg/ml for α-glucosidase which were lower as compared to RC
bark. However, the amount of antioxidants enzymatic as well as non-enzymatic were found to be
higher in RC bark; the IC50 values being lesser; 204.79 μg/ml for DPPH, 192.67 μg/ml for
hydroxyl radical and 188.34 μg/ml for metal chelating activity. These results indicate R. communis
root as a good anti-diabetic agent whereas its bark is having more potent antioxidant system.
Keywords: Diabetes Mellitus, Oxidative stress, Antioxidant, in vitro, Ricinus communis